Journal: Experimental and Therapeutic Medicine
Article Title: miR-146b-5p activation of hepatic stellate cells contributes to the progression of fibrosis by directly targeting HIPK1
doi: 10.3892/etm.2022.11474
Figure Lengend Snippet: Overexpression of HIPK1 effectively decreases the effect of miR-146b-5p in contribution to the progression of HF. (A) Transfection efficiency of lentivirus. (B) Cell viability of HSC-T6. (C) Relative miR-146b-5p, HIPK1, α-SMA, COL1A1 and TGF-β RNA expression. (D) Relative HIPK1, α-SMA, COL1A1 and TGF-β protein expression. * P<0.05; ** P<0.01; *** P<0.001. HIPK1, homeodomain interacting protein kinase 1; NS, no significance; miR, microRNA.
Article Snippet: Lentivirus packaging was performed using the second-generation lentivirus packaging kit (Shanghai GeneChem Co., Ltd.).
Techniques: Over Expression, Transfection, RNA Expression, Expressing